Identification of Schistosoma mansoni glycoproteins recognized by protective antibodies from mice immunized with irradiated cercariae

Technical Report · Sun Jun 15 04:00:00 EDT 1986

OSTI ID:6408797

Dalton, J P; Strand, M; Mangold, B L; Dean, D A

The humoral immune response of mice patently infected with Schistosoma mansoni and of mice vaccinated with radiation-attenuated cercariae were compared by radioimmunoassays and one-and two-dimensional polyacrylamide gel analyses of radioimmunoprecipitates. The binding observed with antibodies of mice vaccinated twice with radiation-attenuated cercariae over a period of 7 to 11 wk was less than 50% of the binding observed with antibodies of mice patently infected for 20 wk, but three to four times greater than that obtained with antibodies of mice infected for 6 wk, irrespective of whether the test extracts were derived from schistosomula or adult worms. Sera of vaccinated mice precipitated a restricted number of predominantly high m.w. glycoproteins of both schistosomula and adult worms metabolically labeled with sulfur-35 methionine. Each of the glycoproteins of 36 hr in vitro-cultured schistosomula that was precipitated by the sera of vaccinated mice was also precipitated by the sera of infected mice. Although radiation-attenuated larvae do not reach the adult stage, mice vaccinated with these still elicit a strong immune response against egg glycoproteins. These results show that the antibody response in mice vaccinated with radiation-attenuated larvae differs qualitatively and quantitatively from that of infected mice.

• Research Organization: Johns Hopkins Univ., Baltimore, MD (USA). School of Medicine
• OSTI ID: 6408797
• Report Number(s): AD-A-179688/7/XAB
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADULTS
AGE GROUPS
ANIMALS
ANTIBODIES
ANTIGENS
CARBOHYDRATES
DRUGS
GLUCOPROTEINS
HELMINTHS
IMMUNE REACTIONS
IMMUNITY
IMMUNOASSAY
IMMUNOLOGY
IRRADIATION
ISOTOPE APPLICATIONS
MAMMALS
MICE
ORGANIC COMPOUNDS
PLATYHELMINTHS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOPROTECTIVE SUBSTANCES
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES