Phytohemagglutinin-induced differentiation and blastogenesis of precursor T cells from mouse bone marrow

Journal Article · Sat Jan 01 04:00:00 EST 1977 · J. Exp. Med.; (United States)

DOI:https://doi.org/10.1084/jem.146.3.735· OSTI ID:5868803

Press, O W; Rosse, C; Clagett, J

The cells in mouse bone marrow (BM) capable of responding to phytohemagglutinin (PHA) were shown to be precursor T cells in experiments employing athymic mice, immunofluorescence, and specific lysis of T or B cells with cytotoxic antisera + complement. In contrast, the responses of lymph node (LN) and spleen (Spl) cells to this mitogen were shown by the same techniques to rely upon resident populations of mature T lymphocytes in these peripheral lymphoid organs. Cytolysis of T cells with anti-theta (anti-Thy 1), anti-thymocyte, or anti-brain antisera abolished the PHA responses of LN and Spl but had no appreciable effect on the BM PHA response. Lysis of B cells with anti-mouse gamma globulin or anti-mouse IgM antisera had no significant effect on either Spl or BM blastogenesis in response to this lectin. Immunofluorescent studies with fluoresceinated anti-brain sera demonstrated acquisition of T-cell surface antigens by BM null lymphocytes during the blastogenic response of this tissue to PHA. The results of these immunofluorescence experiments were reproducible even when marrow obtained from nude mice and pretreated with anti-brain serum plus complement was employed. The implications of these findings with regard to prophylaxis against graft versus host disease in BM transplant recipients are discussed.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 5868803
• Journal Information: J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 146; ISSN JEMEA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIGENS
AROMATICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
COMPLEMENT
CONNECTIVE TISSUE CELLS
DYES
EMISSION SPECTROSCOPY
FLUORESCEIN
FLUORESCENCE SPECTROSCOPY
GRAFT-HOST REACTION
HEMAGGLUTININS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNE SERUMS
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
LYSIS
MAMMALS
MEDICINE
MICE
MITOGENS
MUCOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
PHYTOHEMAGGLUTININ
POLYPHENOLS
POLYSACCHARIDES
PREVENTIVE MEDICINE
PROTEINS
RETICULOENDOTHELIAL SYSTEM
RODENTS
SACCHARIDES
SOMATIC CELLS
SPECTROSCOPY
SPLEEN CELLS
THYMUS
THYMUS CELLS
TISSUES
TRANSPLANTS
VERTEBRATES