Anti-brain antisera bind primarily to non-T cells in mouse bone marrow

Journal Article · Wed Nov 30 23:00:00 EST 1977 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-156-39962· OSTI ID:5135706

Press, O W; Rosse, C; Clagett, J

Anti-brain heteroantisera are widely employed for the delineation of T lymphocytes in lymphomyeloid organs despite compelling evidence demonstrating the cross reactivity of such reagents with unrelated hemopoietic cells. The experiments described in the paper utilized absorptions of a fluoresceinated goat anti-rat brain antiserum with thymocytes or nude bone marrow cells to determine the validity of using such an antiserum to identify T cells in the thymus, lymph nodes, spleen, and bone marrow of CBA/J mice. Thymocyte absorption of this fluoresceinated antiserum removed virtually all of the staining of thymus, lymph node, and spleen cells by this reagent. In contrast, the percentage of labeled marrow cells was unchanged by thymocyte absorption of the anti-rat brain antiserum, although the staining could be eliminated by absorption with nude bone marrow. These results suggest that anti-brain antisera can be employed as dependable T-cell probes with suspensions of the thymus or peripheral lymphoid organs. However, the vast majority of marrow cells staining with the anti-brain antisera are not T cells. Therefore, anti-brain antisera should not be utilized to discern T lymphocytes in this tissue unless additional absorptions (with nude marrow) or special controls are performed.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 5135706
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 156:3; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOASSAY
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CONNECTIVE TISSUE CELLS
IMMUNE SERUMS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
NERVOUS SYSTEM
ORGANS
SKELETON
SOMATIC CELLS
SPLEEN
THYMUS