Differences in lifespan and rate of turnover between phytohemagglutinin responsive cells of the bone marrow and of peripheral lymphoid organs. [Mice]
Technical Report
·
OSTI ID:5371003
Radioautographic analyses were performed on PHA stimulated cultures of in vivo labeled cells obtained from mice previously injected with /sup 3/H-TdR to selectively label either cells with a rapid renewal rate (RR) or a slow renewal rate (SR). PHA responsive cells in the bone marrow (BM) were found to be virtually all RR cells, whereas both RR and SR cells from lymph nodes (LN) and spleen (Spl) were stimulated by this mitogen. However, RR cells were proportionately more responsive to PHA than SR cells in all tissues examined. Only one out of 200 BM PHA blasts belonged to the SR subclass, whereas the RR/SR ratio was approximately 1/1 for LN and 2/1 for Spl. Control experiments demonstrated that significant in vitro reutilization of /sup 3/H-TdR from dying cells did not occur in the cultures. These results support a growing body of evidence that BM PHA responsive cells are precursor T cells which are known to have a rapid turnover rate.
- Research Organization:
- Washington Univ., Seattle (USA)
- OSTI ID:
- 5371003
- Report Number(s):
- RLO/2225/T16-35
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
BONE MARROW
CARBOHYDRATES
CELL PROLIFERATION
HEMAGGLUTININS
HEMATOPOIETIC SYSTEM
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIFE SPAN
LIGHT NUCLEI
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
METABOLISM
MICE
MUCOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN
THYMOCYTES
TISSUES
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
BONE MARROW
CARBOHYDRATES
CELL PROLIFERATION
HEMAGGLUTININS
HEMATOPOIETIC SYSTEM
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIFE SPAN
LIGHT NUCLEI
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
METABOLISM
MICE
MUCOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN
THYMOCYTES
TISSUES
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES