Distribution of rapidly and slowly renewed T, B, and ''Null'' lymphocytes in mouse bone marrow, thymus, lymph nodes, and spleen

Journal Article · Thu Sep 01 04:00:00 EDT 1977 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(77)90139-3· OSTI ID:7294609

Press, O W; Rosse, C; Clagett, J

Combined radioautography and immunofluorescence were employed to discern the proportions of rapidly renewed (RR) and slowly renewed (SR) T, B, and null cells in mouse lymph nodes (LN), spleen (Spl), thymus (Thy), and the lymphocyte-rich fraction of bone marrow (BML). Thy and BML were found to consist predominantly of cells with a rapid turnover rate (95.4 and 76.9 percent, respectively) in accord with their roles as primary lymphoid organs. In contrast, the secondary lymphoid organs were primarily composed of SR cells (LN, 73.4 percent; Spl, 67.8 percent) and contained a more even admixture of the six lymphocyte subsets. Most cells in Thy were RR T cells (93.3 percent), whereas the predominant lymphocyte subpopulation in both LN and Spl consisted of SR T cells (56 and 50 percent, respectively), with RR T cells being much less frequent (14.6 percent LN; 6.8 percent Spl). BML contained both RR and SR cells which stained with the T-cell reagent (7.8 and 7.1 percent). These experiments represent the first comprehensive investigation of the composition of the lymphomyeloid organs in terms of RR and SR T, B, and null cells. They conclusively demonstrate RR and SR lymphocytes in all three functional categories (T, B, and null) and show characteristic tissue distributions of the six lymphocyte subsets.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 7294609
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 33:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISTRIBUTION
FLUORESCENCE
HEMATOPOIETIC SYSTEM
LEUKOCYTES
LUMINESCENCE
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MICE
ORGANS
RODENTS
SOMATIC CELLS
SPLEEN
THYMOCYTES
THYMUS
TISSUES
VERTEBRATES