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Isobologram analysis of X-ray-BCNU interactions in vitro

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575176· OSTI ID:5868059
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In this study, 9L rat brain tumor cells were exposed for 1 hr to either 1, 3, 5, or 7.5 ..mu..g/ml 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), followed 15 hr later with graded doses (0 to 20 Gy) of radiation. Isobolograms constructed for 1, 2, and 3 log cell kills showed that lethality from BCNU and from radiation was additive. The analysis was extended to lower levels of survival by constructing additive dose-response curves from the isobolograms. These curves define the additive, subadditive, supraadditive, and protective regions for each survival plot. For log cell kills greater than 3, additivity of lethality was found for radiation and either 1 or 7.5 ..mu..g/ml BCNU, while supraadditivity of lethality was found for radiation and either 3 or 5 ..mu..g/ml BCNU. Therefore, all concentrations of BCNU interacted positively with all doses of radiation to give enhanced cell kill.
Research Organization:
Univ. of California, San Francisco
OSTI ID:
5868059
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 79:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BODY
BRAIN
CELL KILLING
CENTRAL NERVOUS SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
IN VITRO
IONIZING RADIATIONS
MAMMALS
MATHEMATICAL MODELS
NERVOUS SYSTEM
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATIONS
RATS
RODENTS
SURVIVAL CURVES
SYNERGISM
VERTEBRATES
X RADIATION