Effects of nickel compounds on incorporation of (/sup 3/H) thymidine into DNA in rat liver and kidney

Journal Article · Mon Mar 31 23:00:00 EST 1980 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/1.4.297· OSTI ID:5841256

Hui, G; Sunderman, Jr, F W

In vivo incorporation of (/sup 3/H) thymidine into DNA was determined in rats at 28 h after partial hepatectomy. Administration of nickel carbonyl (Ni(CO)/sub 4/) at 2 or 4 h before sacrifice inhibited (/sup 3/H) thymidine uptake into liver and kidney DNA. For example, in rats killed 4 h after i.v. injection of Ni(CO)/sub 4/ (2 mg Ni/100 g), (/sup 3/H)-labelling of liver DNA averaged 54 (SE +- 10% of controls (p<0.05), and (/sup 3/H)-labelling of kidney DNA averaged 53 (SE +- 6)% of controls (p<0.01). Injection of NiCl/sub 2/(2 mg Ni/100 g, i.m.) 4 h before death did not significantly affect (/sup 3/H) thymidine uptake into liver DNA, but did inhibit (/sup 3/H) thymidine uptake into kidney DNA (65 +- 6%, p<0.02). Binding of /sup 63/Ni to DNA in liver and kidney of rats killed 4 h after injection of /sup 63/Ni(CO)/sub 4/ or /sup 63/NiCl/sub 2/ ranged from 0.3 to 2.2 mol /sup 63/Ni/mol of DNA nucleotides. Ultracentrifugation of DNA on alkaline sucrose gradients did not reveal any differences between sedimentation profiles of hepatic DNA from Ni(CO)/sub 4/-treated rats versus paired control rats.

• Research Organization: Univ. of Connecticut, Farmington
• OSTI ID: 5841256
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 1; ISSN CRNGD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CENTRIFUGATION
DIGESTIVE SYSTEM
DNA
EVEN-ODD NUCLEI
GLANDS
HETEROCYCLIC COMPOUNDS
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
NICKEL 63
NICKEL COMPOUNDS
NICKEL ISOTOPES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RATS
RIBOSIDES
RODENTS
SEPARATION PROCESSES
SYNTHESIS
THYMIDINE
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES
YEARS LIVING RADIOISOTOPES