Effects of nickel compounds on incorporation of (/sup 3/H) thymidine into DNA in rat liver and kidney

Journal Article · Mon Mar 31 23:00:00 EST 1980 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/1.4.297· OSTI ID:5627581

Hui, G; Sunderman, Jr, F W

In vivo incorporation of (/sup 3/H) thymidine into DNA was determined in rats at 28 h after partial hepatectomy. Administration of nickel carbonyl (Ni(CO)/sub 4/) at 2 or 4 h before sacrifice inhibited (/sup 3/H) thymidine uptake into liver and kidney DNA. Injection of NiCl/sub 2/ 4 h before death did not significantly affect (/sup 3/H) thymidine uptake into liver DNA, but did inhibit (/sup 3/H) thymidine uptake into kidney DNA. Binding of /sup 63/Ni to DNA in liver and kidney of rats killed 4 h after injection of /sup 63/Ni(CO)/sub 4/ or /sup 63/NiCl/sub 2/ ranged from 0.3 to 2.2 mol /sup 63/Ni/mol of DNA nucleotides. Ultracentrifugation of DNA on alkaline sucrose gradients did not reveal any differences between sedimentation profiles of hepatic DNA from Ni(CO)/sub 4/-treated rats versus paired control rats.

• Research Organization: Univ. of Connecticut, Farmington
• DOE Contract Number: AC02-76EV03140
• OSTI ID: 5627581
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 1; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBONYLS
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
DNA
EVEN-ODD NUCLEI
GLANDS
HALIDES
HALOGEN COMPOUNDS
HEPATECTOMY
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEDICINE
NICKEL 63
NICKEL CHLORIDES
NICKEL COMPOUNDS
NICKEL ISOTOPES
NICKEL SULFIDES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RATS
RIBOSIDES
RODENTS
SULFIDES
SULFUR COMPOUNDS
SURGERY
SYNTHESIS
THYMIDINE
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES