Induction of mutagenesis and transformation in BALB/c-3T3 clone A31-1 cells by diverse chemical carcinogens

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850160103· OSTI ID:5840058

Lubet, R A ^[1]; Kouri, R E; Curren, R A; Putman, D L; Schechtman, L M ^[2]

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1. National Cancer Institute, Frederick, MD (USA)
2. Microbiological Associates, Bethesda, MD (USA)

BALB/c-3T3 cells were employed to examine the genotoxic potential of a variety of known chemical carcinogens. BALB/c-3T3 cells displayed a dose-dependent transformation response to a variety of carcinogens (polycyclic hydrocarbons, methylating agents, ethylating agents, aflatoxin B{sub 1} (AFT{sub 1}), and 4-nitroquinoline-N-oxide (4-NQO)). When the ability of these compounds to induce mutagenesis to resistance to the cardiac glycoside ouabain (OUA{sup R}) was examined, the authors found the short chain alkylating agents to be particularly effective mutagens, causing biologic effects at doses below those necessary to induce a transformation response. In contrast, the polycyclic hydrocarbons which were potent transforming agents were weaker, albeit significant, mutagens for the OUA{sup R} locus in this system, while AFB{sub 1} was quite weak. Further studies were performed with 5-azacytidine (5-AZA) and the nongenotoxic carcinogen cinnamyl anthranilate (CIN). 5-AZA was a potent transforming agent, but failed to cause mutagenesis. CIN similarly caused in vitro transformation. When a series of eight structurally diverse compounds were examined in both the BALB/c-3T3 and C3H10T1/2 mouse fibroblast transformation systems, the BALB/c-3T3 system was shown to be sensitive to a wide variety of potential carcinogens, whereas the C3H10T1/2 system proved routinely sensitive only to the polycyclic hydrocarbons.

• OSTI ID: 5840058
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 16:1; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXINS
ANIMAL CELLS
ANTIGENS
AROMATICS
AZAARENES
AZINES
BENZOPYRENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL TRANSFORMATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
FIBROBLASTS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
MATERIALS
MUTAGENESIS
NITRO COMPOUNDS
NITROSO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
POLYCYCLIC NITRO COMPOUNDS
PYRIDINES
QUINOLINES
SOMATIC CELLS
TOXIC MATERIALS
TOXINS