Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, August 1, 1977-October 31, 1980
The objective of this research is to determine the role of DNA repair in mutagenesis and carcinogenesis in mammalian cells. More specifically, mutant strains will be selected which are deficient in various DNA repair pathways. These strains will be studied with regard to (1) the nature of the defect in repair, and (2) the mutability and transformability of the defective cells by various agents as compared to the wild type parental cells. The results to date include progress in the following areas: (1) determination of optimum conditions for growth and maintenance of cells and for quantitative measurement of various cellular parameters; (2) investigation of the effect of holding mutagenized cells for various periods in a density inhibited state on survival and on mutation and transformation frequencies; (3) examination of the repair capabilities of BHK cells, as compared to repair-proficient and repair-deficient human cells and excision-deficient mouse cells, as measured by the reactivation of Herpes simplex virus (HSV) treated with radiation and ethylmethane sulfonate (EMS); (4) initiation of host cell reactivation viral sucide enrichment and screening of survivors of the enrichment for sensitivity to ionizing radiation; and (5) investigation of the toxicity, mutagenicity, and carcinogenicity of various metabolites of 4-nitroquinoline-1-oxide (4-NQO). (ERB)
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH (USA)
- DOE Contract Number:
- AS02-77EV04472
- OSTI ID:
- 6760273
- Report Number(s):
- DOE/EV/04472-4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1979-October 31, 1980
Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Three-year report, February 1, 1981-September 30, 1983
Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1980-October 31, 1981
Technical Report
·
Wed Oct 01 00:00:00 EDT 1980
·
OSTI ID:6740147
Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Three-year report, February 1, 1981-September 30, 1983
Technical Report
·
Fri Sep 30 00:00:00 EDT 1983
·
OSTI ID:5671458
Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1980-October 31, 1981
Technical Report
·
Thu Oct 01 00:00:00 EDT 1981
·
OSTI ID:5296815
Related Subjects
550200 -- Biochemistry
550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
CARCINOGENESIS
CELL CULTURES
COMPARATIVE EVALUATIONS
CORRELATIONS
CYTOLOGICAL TECHNIQUES
DNA
GENETIC VARIABILITY
HOST-CELL REACTIVATION
METABOLITES
MICROORGANISMS
MUTAGENESIS
MUTAGENS
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
RECOVERY
REPAIR
TOXICITY
VIRUSES
550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
CARCINOGENESIS
CELL CULTURES
COMPARATIVE EVALUATIONS
CORRELATIONS
CYTOLOGICAL TECHNIQUES
DNA
GENETIC VARIABILITY
HOST-CELL REACTIVATION
METABOLITES
MICROORGANISMS
MUTAGENESIS
MUTAGENS
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
RECOVERY
REPAIR
TOXICITY
VIRUSES