Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Three-year report, February 1, 1981-September 30, 1983

Technical Report · Fri Sep 30 04:00:00 EDT 1983

OSTI ID:5671458

Evans, H H

Mutant strains were selected which are deficient in various DNA repair pathways and these were studied with regard to (1) the nature of the defect in repair, and (2) the mutability and transformability of the defective cells by various agents as compared to the wild type parental cells. Lightly mutagenized wild-type cells were infected with irradiated herpes simplex virus (HSV). Cells which repair HSV are lysed so the surviving population is enriched in repair-deficient cells. Six strains which survived two rounds of infection were characterized with respect to their radiosensitivity.

• Research Organization: Case Western Reserve Univ., Cleveland, OH (USA)
• DOE Contract Number: AC02-77EV04472
• OSTI ID: 5671458
• Report Number(s): DOE/EV/04472-T3; ON: DE84000837
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARCINOGENESIS
CELL CULTURES
CHROMATOGRAPHY
CONTROL
DISEASES
DNA
DOSE RATES
ELECTROMAGNETIC RADIATION
EMS
ESTERS
GENETIC CONTROL
GUANINE
HERPES SIMPLEX
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MUTAGENESIS
MUTAGENS
NITROSAMINES
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
PEST CONTROL
PURINES
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SEPARATION PROCESSES
SKIN DISEASES
STRAND BREAKS
SULFONIC ACID ESTERS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VIRAL DISEASES
X RADIATION