Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1980-October 31, 1981

Technical Report · Thu Oct 01 04:00:00 EDT 1981

OSTI ID:5296815

Evans, H H

The isolation of several radiation-sensitive BHK strains following a host-cell viral suicide enrichment procedure has been reported in which mutagenized cells were infected with heavily irradiated Herpes virus (HSV). Six surviving colonies were isolated from 38,000 infected cells. The survivors were not transformed by HSV, as indicated by a lack of reaction with fluorescent HSV antibody. At least two of the strains were shown to be sensitive to the lethal effects of ionizing radiation and methylmethane sulfonate, but not to ethylmethane sulfonate (EMS) or to uv radiation. These two strains showed a small decrease in the ability to repair sublethal damage following a split dose of ionizing radiation. The two strains differed from wild-type BHK cells in EMS-induced mutability; strain VI showed a higher mutation frequency and V2 a lower mutation frequency than did BHK cells following treatment with this agent. When either ionizing radiation or uv radiation was used as the mutagenic agent, however, the comparative mutability patterns were altered: the mutation frequency of both strains was somewhat less than the wild type following ionizing radiation, whereas following uv radiation, strain V1 showed a markedly lower mutation frequency than the wild type. It is possible that the strain V1 is deficient in the repair of an EMS-induced mutagenic lesion, while strain V2 is either efficient in such repair or deficient in an error-prone repair process.

• Research Organization: Case Western Reserve Univ., Cleveland, OH (USA)
• DOE Contract Number: AS02-77EV04472
• OSTI ID: 5296815
• Report Number(s): DOE/EV/04472-3-81; ON: DE82007958
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
DNA
ELECTROMAGNETIC RADIATION
EMS
ESTERS
HAMSTERS
IONIZING RADIATIONS
KIDNEYS
MAMMALS
METHYL METHANESULFONATE
MUTAGENESIS
MUTAGENS
MUTATION FREQUENCY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SENSITIVITY
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
VERTEBRATES