Regulation of monocyte oxidative metabolism: chemotactic factor enhancement of superoxide release, hydroxyl radical generation, and chemiluminescence

Journal Article · Wed Nov 30 23:00:00 EST 1983 · J. Lab. Clin. Med.; (United States)

OSTI ID:5830905

Janco, R L; English, D

Human peripheral blood mononuclear cells exposed to the synthetic chemotactic factor n-formyl-methionyl-leucyl-phenylalanine (FMLP) were enhanced in their ability to generate superoxide anion (O/sub 2//sup -/), hydroxyl radical (OH), and chemiluminescence when later exposed to phorbol myristate acetate (PMA). When compared to oxidative responses of cells treated with PMA alone, the degree of enhancement by pretreatment with FMLP was 1.85-fold for O/sub 2//sup -/ generation, 1.73-fold for OH production, and 1.34-fold for chemiluminescence. Similarly, pretreatment of mononuclear leukocytes with 5% zymosan-activated serum also enhanced subsequent oxidative responses of cells exposed to PMA. FMLP did not enhance subsequent O/sub 2//sup -/ release or chemiluminescence by mononuclear leukocytes stimulated by opsonized zymosan or 20 mM sodium fluoride (F/sup -/), demonstrating that the O/sub 2//sup -/ generating system of monocytes stimulated by phagocytosis or F/sup -/ is not susceptible to chemotactic factor regulation in a manner similar to the system stimulated by PMA. The latter system, like that of neutrophils, is susceptible to regulation by cellular processes activated during an initial encounter with chemoattractants. These processes may provide a mechanism to amplify oxidative responses at sites of infection or inflammation, leading to enhanced efficiency of microbial activity or increased tissue damage in vivo. 34 references, 2 figures, 2 tables.

• Research Organization: Vanderbilt Univ. Medical Center, Nashville, TN
• OSTI ID: 5830905
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 102:6; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHARGED PARTICLES
CHEMILUMINESCENCE
DATA
ESTERS
EXPERIMENTAL DATA
HYDROXYL RADICALS
INFORMATION
IONS
LEUKOCYTES
LUMINESCENCE
MATERIALS
METABOLISM
METABOLITES
MONOCYTES
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHORBOL ESTERS
PHYSIOLOGY
RADICALS
RESPONSE MODIFYING FACTORS
TOXICITY