Effect of a high molecular weight hyaluronic acid (HA) preparation on the stimulation of polymorphonulcear leukocytes (PMNL)

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5006414

McNeil, J; Chow, D C; Skosey, J L

During the process of joint inflammation PMNL are attracted into the joint space by chemotactic agents and are stimulated by immune complexes, particular matter (eg, crystals, cartilage debris) and other phlogistic agents. This process occurs in an environment rich in HA. The authors have examined the effect of high molecular weight HA. They have examined the effect of high molecular weight HA upon PMNL stimulation. PMNL were isolated from human blood and stimulated with either opsonized zymosan or formyl-methionyl-leucyl-phenylalanine (fmlp). The authors assessed stimulation by measuring the ability of cell supernatants to promote the release of /sup 35/S from chips of rabbit articular cartilage labeled in vivo, and the enhancement of oxidation of (1-/sup 14/C)glucose to /sup 14/CO/sub 2/. Stimulation of cells with zym in the presence of HA, 0.125-2.5 mg/ml, resulted in enhanced /sup 35/S release (33-59% over zym alone) and /sup 14/CO/sub 2/ production (0.5-64%). However, HA failed to enhance responses when fmlp (+cytochalasin B) was used as the stimulus. It has been demonstrated that high molecular weight HA inhibits phagocytosis of both latex and aggregated IgG. In our studies, it is likely that HA interference with ingestion of zym leads to frustrated phagocytosis and enhancement of PMNL responses. Similar modification of responses of inflammatory mediator cells could occur in inflamed joints.

• Research Organization: Univ. of Illinois College of Medicine, Chicago
• OSTI ID: 5006414
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE JOINTS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLUCOSE
HEXOSES
HYALURONIC ACID
IMMUNE REACTIONS
INFLAMMATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
MOLECULAR WEIGHT
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHAGOCYTOSIS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SKELETON
SULFUR 35
SULFUR ISOTOPES
SYMPTOMS
TRACER TECHNIQUES