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Superoxide generation and cytotactic response of irradiated neutrophils

Journal Article · · Transfusion (Philadelphia); (United States)
;

Irradiation of blood components has been used to prevent transfusion-related graft-versus-host disease (GVHD) in immunocompromised patients. This study was designed to determine the effect of irradiation on neutrophil aggregation, chemotaxis, and superoxide generation. Purified neutrophils were irradiated with a Cesium source at four doses ranging from 0 to 17,500 rads. Formyl-methionyl-leucyl-phenylalanine (FMLP) and zymosan-treated serum (ZTS) cytotaxin-induced chemotaxis and migration were determined in the agarose assay. Neutrophil aggregation to FMLP was determined by aggregometry. Superoxide generation and random migration were not affected by irradiation at doses up to 17,500 rads. When compared to nonirradiated controls, the chemotactic response to ZTS remained normal, with an insignificant decline from 174 +/- 31.0 to 150 +/- 42.3 (mean +/- SD) units. The chemotactic response to FMLP declined insignificantly, from 228 +/- 31.3 at 0 rad to 207 +/- 26.4 at 17,500 rads. The aggregation response to FMLP remained within the normal range but declined from 0.78 +/- 0.11 to 0.61 +/- 0.18. At the radiation doses currently used to reduce the risk of transfusion-related GVHD, neutrophil superoxide generation and chemotactic response remain essentially normal.

Research Organization:
Northeastern New York Region, Albany (USA)
OSTI ID:
6823889
Journal Information:
Transfusion (Philadelphia); (United States), Journal Name: Transfusion (Philadelphia); (United States) Vol. 28:4; ISSN TRANA
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLOMERATION
AMINO ACIDS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
IMMUNE REACTIONS
LEUKOCYTES
MATERIALS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTOSIS
PHENYLALANINE
RADIATION EFFECTS
RADICALS
SUPEROXIDE RADICALS
SYNTHESIS
THERAPY
TRANSFUSIONS
ZYMOSAN