Bronchial epithelial cells release monocyte chemotactic activity in response to smoke and endotoxin
- Research Service, Omaha Veterans Administration Medical Center, NE (USA)
An increase in mononuclear phagocytes occurs within the airways during airway inflammation. Bronchial epithelial cells could release monocyte chemotactic activity and contribute to this increase. To test this hypothesis, bovine bronchial epithelial cells were isolated and maintained in culture. Bronchial epithelial cell culture supernatant fluids were evaluated for monocyte chemotactic activity. Epithelial cell culture supernatant fluids attracted significantly greater numbers of monocytes compared to media alone and the number of monocytes attracted increased in a time dependent manner. Endotoxin and smoke extract induced a dose and time dependent release of monocyte chemotactic activity compared with cells cultured in media (52.5 {plus minus} 2.6 (endotoxin), 30.5 {plus minus} 2.3 (smoke) vs 20.5 {plus minus} 2.2 cells/high power field (HPF) p less than 0.001). The released activity was chemotactic by checkerboard analysis. Stimulation of the epithelial cells by opsonized zymosan, calcium ionophore (A23187), and PMA also resulted in an increase in monocyte chemotactic activity (p less than 0.01). Because the release of activity was blocked by the lipoxygenase inhibitors, nordihydroguaiaretic acid and diethycarbamazine, epithelial cell monolayers were cultured with 3 microCi (3H)arachidonic acid for 24 h and then exposed to A23187, PMA, or both stimuli, for 4, 8, and 24 h. Analysis of the released 3H activity was performed with reverse-phase HPLC and revealed that the major lipoxygenase product was leukotriene B4. These data suggest that monocytes may be recruited into airways in response to chemotactic factors released by bronchial epithelial cells.
- OSTI ID:
- 5226575
- Journal Information:
- Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 147:3; ISSN 0022-1767; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
AEROSOLS
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BRONCHI
CARBOXYLIC ACIDS
CARCINOGENS
CATTLE
CELL CULTURES
CHROMATOGRAPHY
COLLOIDS
CORTICOSTEROIDS
CYCLOHEXIMIDE
DISPERSIONS
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENDOTOXINS
ENZYME INHIBITORS
ENZYMES
EPITHELIUM
ESTERS
FUNGICIDES
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KETONES
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
MONOCYTES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PESTICIDES
PHAGOCYTOSIS
PHORBOL ESTERS
PREGNANES
RESIDUES
RESPIRATORY SYSTEM
RUMINANTS
SENSITIVITY
SEPARATION PROCESSES
SMOKES
SOLS
STEROID HORMONES
STEROIDS
TIME DEPENDENCE
TISSUES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES