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PAF polar head group heterogeneity: effects of human neutrophil (PMN) stimuli

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6897019
; ; ;

Platelet-activating factor (PAF) is a potent phospholipid produced by stimulated human polymorphonuclear leukocytes (PMN). Tritiated-PAF produced by human PMN (5 x 10/sup 6/ cells/ml) stimulated in the presence of /sup 3/H-acetate was separated by normal phase HPLC. PMN stimulated with the calcium ionophore A23187 (2.5 ..mu..M, final concentration) produced two peaks of /sup 3/H-activity both of which activated platelets; the major peak (92.3% of total cpm) had the same relative retention time (RRT) as 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (C16:0-AGEPC, RRT = 1.00) when related to the retention time of the internal standard /sup 3/HC16:O-AGEPC while the smaller peak (2.4%) had a RRT of 0.61. In contrast, PMN stimulated with the chemotactic peptide N'formyl-methionyl-leucyl-phenyl-alanine (FMLP; 10/sup -6/M), opsonized zymosan (1.8 mg/ml), or phorbol myristate acetate (PMA; 20 ng/ml) produced /sup 3/H-PAG that was separated into 5 peaks of /sup 3/H-activity (RRT = 0.23, 0.36, 0.65, 1.00 and 1.23). PAF-2-acyl-hydrolase released /sup 3/H-acetate from the molecules with RRT of 1.00 and 1.23 but not 0.65, indicating that the latter molecule likely would be the ethanolamine analogue. Thus, the current findings indicate that the PAF produced by stimulated human PMN is comprised of molecules containing polar head groups other than choline and the quantity and quality appear to be regulated by the different types of PMN stimuli.

Research Organization:
Univ. of Texas Health Science Center, San Antonio
OSTI ID:
6897019
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY FLUIDS
CARBOXYLIC ACID SALTS
CARCINOGENS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
COAGULANTS
DRUGS
ESTERS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
NEUTROPHILS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
PRIMATES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES