Polar head and SN-1 chain group heterogeneity in PAF-like molecules synthesized by ionophore-stimulated human neutrophils (PMN)

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6942977

Ludwig, J C; Lear, C; McManus, L M; Pinckard, R N

Platelet-activating factor (PAF) was synthesized and released by human polymorphonuclear leukocytes (PMN; 5 x 10/sup 6/ ml) stimulated in the presence of /sup 3/H-acetate with the calcium ionophore A23187 (2.5 ..mu..M). The temporal incorporation of /sup 3/H-acetate into phospholipids by stimulated PMN occurred concomitantly with PAF production. Phospholipid separation of cell-associated PAF by normal phase HPLC revealed the presence of 2 peaks of /sup 3/H-activity both possessing platelet stimulating activity. The major peak of /sup 3/H-PAF coeluted with 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) while the smaller peak coeluted with 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoethanolamine/dimethylethanolamine/monomethylethanolamine. Reverse phase HPLC fractionation of the PAF activity in the choline containing region indicated the presence of 15 peaks of /sup 3/H-activity, all of which exhibited PAF activity. In addition, reverse phase HPLC of the PAF in the ethanolamine-containing region resolved an additional 6 peaks of /sup 3/H-activity. Moreover, these PAF molecules differed in their potencies to activate platelets. Thus, the current findings suggest that alterations in the distribution of the 21 PAF-like molecules synthesized and released by stimulated human PMN could modulate the pathogenesis of PAF-mediated tissue injury.

• Research Organization: Univ. of Texas Health Science Center, San Antonio
• OSTI ID: 6942977
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETATES
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY
BODY FLUIDS
CARBOXYLIC ACID SALTS
CHROMATOGRAPHY
COAGULANTS
DRUGS
ESTERS
HEMATOLOGIC AGENTS
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
MOLECULES
NEUTROPHILS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PRIMATES
SEPARATION PROCESSES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES