Quantitative autoradiography with radiopharmaceuticals, Part 2: Applications in radiopharmaceutical research: concise communication

Journal Article · Mon Feb 28 23:00:00 EST 1983 · J. Nucl. Med.; (United States)

OSTI ID:5821772

Som, P; Yonekura, Y; Oster, Z H; Meyer, M A; Pelletteri, M L; Fowler, J S; MacGregor, R R; Russell, J A; Wolf, A P; Fand, I

We describe the application of macroautoradiography, a relatively simple, quantifiable method for the evaluation of positron-emitting and gamma-emitting radiopharmaceuticals. We have investigated the response properties of two types of film to positron (F-18) and negatron (C-14) emitters. Variations in the response of film to increasing film-to-source distance are described, along with the effects of different intensifying screens and mounting tape. Digitization of whole-body autoradiograms (WBARG) in small animals was performed by using a videodensitometry system (videocamera interfaced to a computer). Quantitation was derived from analysis of a series of step-wedge standards that covered the range of radioactivities in the sample. By using a close-up lens on the videocamera, a 2- by 2-cm field is digitized as a 128 X 128 array, each pixel representing 156 X 156 micron. The effect of chlorpromazine (CPZ) on glucose metabolism in mice was studied by giving C-14 2DG followed by CPZ and F-18 FDG in the same animal. Muscle activity decreased and brown-fat activity increased. The high spatial resolution of this technique enables quantification in structures as small as the basal ganglia in mice. The use of dual-nuclide ARG permits each animal to be its own control, which greatly increases the utility of this method.

• Research Organization: Brookhaven National Laboratory, Upton, New York
• OSTI ID: 5821772
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 24:3; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
CAMERAS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM AGENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHLORPROMAZINE
DISPLAY DEVICES
DRUGS
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HYPNOTICS AND SEDATIVES
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOTHIAZINES
PSYCHOTROPIC DRUGS
RADIOPHARMACEUTICALS
RATS
RODENTS
SACCHARIDES
TRANQUILIZERS
USES
VERTEBRATES