Cross linking in the radiolysis of some enzymes and related proteins

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Adv. Exp. Med. Biol.; (United States)

DOI:https://doi.org/10.1007/978-1-4684-3282-4_33· OSTI ID:5733543

Lynn, K R

In non-covalently bound complexes of several serine proteases and of ribonuclease with DNA the enzymes were protected against the effects of ionizing radiation. No scavenging by the nucleic acids was observed. Similarly, complexing trypsin with silica protected the enzyme from radiolytic destruction. Irradiation of solutions of serine proteases required about twice the D37 dose to produce about 10% polymerization: significantly lower relative doses were effective in causing polymerization in both lima bean protease inhibitor and in the octapeptidal hormone oxytocin. Several sulfhydryl enzymes which have been examined were very efficiently inactivated by ionizing radiation. There was, at the same time, apparent formation of novel intra-molecular -S-S- bonds.

• Research Organization: National Research Council of Canada, Ottawa, Ontario
• OSTI ID: 5733543
• Journal Information: Adv. Exp. Med. Biol.; (United States), Journal Name: Adv. Exp. Med. Biol.; (United States) Vol. 86A; ISSN AEMBA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHALCOGENIDES
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
COMPARATIVE EVALUATIONS
DECOMPOSITION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYMES
HORMONES
HYDROLASES
MINERALS
OXIDES
OXYGEN COMPOUNDS
OXYTOCIN
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PHOSPHOTRANSFERASES
PITUITARY HORMONES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOLYSIS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RESPONSE MODIFYING FACTORS
RNA-ASE
SILICA
SILICON COMPOUNDS
SILICON OXIDES
TRANSFERASES
TRYPSIN