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Enzyme inactivation by peroxyacetyl nitrate

Journal Article · · Arch. Biochem. Biophys.; (United States)
Isocitric dehydrogenase (NADP linked), G-6-P dehydrogenase, and malic dehydrogenase were inactivated by peroxyacetyl nitrate. The enzymes could be protected in some cases; isocitric dehydrogenase by isocitrate and NAPD, the former being more effective, and G-6-P dehydrogenase by NADP but not by G-6-P. Malic dehydrogenase was not significantly protected either by substrate or coenzyme. Inhibition of these three enzymes by cadmium ion or p-chloromercuribenzoate was prevented in the same order by the presence of substrate or coenzyme. The conclusion that peroxyacetyl nitrate inactivated the enzymes by oxidizing sulfhydryl groups was further supported by the resistance of ribonuclease, containing no sulfhydryl groups, to peroxyacetyl nitrate. 17 references, 6 figures.
Research Organization:
Univ. of California, Riverside
OSTI ID:
5929422
Journal Information:
Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 102:1; ISSN ABBIA
Country of Publication:
United States
Language:
English