Inhibitory effect of peroxyacetyl nitrate on cyclic photophosphorylation by chloroplasts from Black Valentine bean leaves
The inhibitory effect of peroxyacetyl nitrate on the cyclic photophosphorylation of chloroplasts isolated from Black Valentine variety bean leaves (Phaseolis vulgaris L.) has been studied. Peroxyacetyl nitrate caused inhibition to photophosphorylation, in either the dark or the light, by affecting the chloroplast. Evidence is presented which suggests that peroxyacetyl nitrate could oxidize sulfhydryl groups on enzymes necessary for photophosphorylation. The inhibition to photophosphorylation caused by peroxyacetyl nitrate cannot be reversed by glutathione, even when added in large amounts, whereas the inhibition to photophosphorylation caused by para-chloromercuriphenylsulfonic acid is easily reversed by small quantities of glutathione. This suggests that if peroxyacetyl nitrate is oxidizing sulfhydryl groups necessary for photophosphorylation, this oxidation is proceeding beyond the disulfide stage. 12 references, 1 figure, 2 tables.
- Research Organization:
- Univ. of California, Riverside
- OSTI ID:
- 5721031
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 42; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLOROPLASTS
DRUGS
ENZYMES
ESTERS
GLUTATHIONE
INHIBITION
LEAVES
LEGUMINOSAE
NITRATES
NITRIC ACID ESTERS
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
PEPTIDES
PEROXYACETYL NITRATE
PHASEOLUS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
POLYPEPTIDES
PROTEINS
RADICALS
RADIOPROTECTIVE SUBSTANCES
SULFHYDRYL RADICALS
SYNTHESIS