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Inhibition of cyclic photophosphorylation by peroxyacetyl nitrate

Journal Article · · Plant Physiol.; (United States)
OSTI ID:5264555

The present paper describes the inhibitory effect of PAN on cyclic photophosphorylation by chloroplasts isolated from Black Valentine bean leaves. If the photophosphorylation reaction mixture is gassed with PAN during the assay, inhibition of photophosphorylation results. Moreover, inhibition of photophosphorylation is also observed if the complete reaction mixture containing the chloroplasts is gassed in complete darkness with PAN immediately before but not during the photophosphorylation assay. The second observation is of considerable interest in view of the fact that intact bean plants do not damage from PAN fumigation unless light is present before, during and after the fumigation. Inhibition of photophosphorylation by PAN gassing of the chloroplasts either as the assay is performed or in the dark prior to the assay is caused by catalytic amounts of PAN. Both types of gassing result in an increase in inhibition which is linear with respect to the increase in PAN. However, the quantity required to cause a given degree of inhibition by gassing during the assay is greater than the quantity required to cause a similar degree of inhibition as a result of dark gassing. That this is not an indirect effect has not been completely ruled out.

Research Organization:
Univ. of California, Riverside
OSTI ID:
5264555
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 38; ISSN PLPHA
Country of Publication:
United States
Language:
English