ESR of /sup 125/IUdR-labeled cells and x-irradiated cells at 4. 2/sup 0/K and above

Journal Article · Mon Oct 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575113· OSTI ID:5733402

Johnson, J E; Moulton, G C; Finch, L L

The high toxicity of /sup 125/I-deoxyuridine (/sup 125/IUdR) to cells was studied by comparing the electron spin resonance (ESR) spectra of cells labeled with /sup 125/IUdR and cells that have been irradiated with x rays. The kinds of radicals produced by the two types of radiation appear to be the same, but there are differences in relative amounts in three cases: (1) several times as many carbon-based radicals relative to the amount of radiation products of cellular water are produced in the /sup 125/I-labeled cells as in the x-irradiated cells. This indicates that the indirect effect due to the radiation products of water are probably of less importance in radiation cell lethality of /sup 125/I-labeled cells than the direct effects; (2) there is more of a 17-G doublet present in the ESR spectra of /sup 125/IUdR-labeled cells than in the x-irradiated cells. This doublet is probably due to the thymine anion radical; and (3) there is more of a radical, which is probably the thymine H-addition radical, present in the /sup 125/IUdR-labeled cells. These results strengthen the hypothesis that the high toxicity of /sup 125/IUdR is due to a high local ionization density at the DNA.

• Research Organization: Florida State Univ., Tallahassee
• OSTI ID: 5733402
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 80:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL KILLING
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
DEOXYURIDINE
DNA
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON SPIN RESONANCE
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONIZING RADIATIONS
ISOLATED VALUES
ISOTOPES
L CELLS
LABELLED COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
MICE
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
QUANTITY RATIO
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESONANCE
RIBOSIDES
RODENTS
SPECTRA
TEMPERATURE EFFECTS
TOXIC MATERIALS
ULTRALOW TEMPERATURE
URACILS
VERTEBRATES
X RADIATION