Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Inhomogeneity of the nucleus to /sup 125/IUdR cytotoxicity

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576434· OSTI ID:5520506
Synchronized suspension cultures of Chinese hamster ovary (CHO) cells were used to determine the lethal effects produced by the decay of /sup 125/I incorporated into different subfractions of the nuclear genome. Such a shift in nuclear incorporation pattern was achieved by using the drug aphidicolin, which inhibits 95% of all nuclear DNA synthesis, is nontoxic to cells in a colony-forming assay, and does not modify the radiation response of CHO cells to X irradiation. These data in combination with survival curves of CHO cells labeled with /sup 125/I-iododeoxyuridine (/sup 125/IUdR) either with or without aphidicolin showed a dramatic change in the survival response (D/sub 0/: 30 decays/cell and 96 decays/cell, respectively). It is concluded, therefore, that the nucleus is not a homogeneous target for radiation-induced cell death because when subfractions of the nuclear genome are labeled, radically different levels in cell survival are obtained.
Research Organization:
Univ. of Utah Medical Center, Salt Lake City
OSTI ID:
5520506
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:1; ISSN RAREA
Country of Publication:
United States
Language:
English