Target for radiation-induced division delay

Journal Article · Mon Dec 01 04:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575485· OSTI ID:6984334

Schneiderman, M H ^[1]; Hofer, K G

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1. Thomas Jefferson Univ. Hospital, Philadelphia, PA

In an effort to elucidate the subcellular target responsible for radiation-induced division delay, Chinese hamster ovary (CHO) cells growing in monolayer cultures were pulse-labeled with /sup 125/IUdR and the cell kinetics monitored by counting the mitotic cells selected every 10 min. Our results showed that /sup 125/I had to be incorporated into DNA to cause a perturbation of cell progression; unlabeled G/sub 2/ cells were unperturbed. To evaluate the mechanism of /sup 125/I-induced division delay, /sup 125/IUdR-labeled cells were permitted to accumulate /sup 125/I decays either during the S phase or during both S and G/sub 2/ phases. The results indicated that cells which accumulated /sup 125/I decays only during the S phase did not experience enhanced delay. In contrast, the yield of mitotic cells was reduced in cells which accumulated /sup 125/I decays during S plus G/sub 2/. Analysis of the data suggests that the target for radiation-induced mitotic delay is not the DNA, but a cell structure which comes in contact with the DNA during G/sub 2/ or early M phase.

• OSTI ID: 6984334
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 84:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL DIVISION
DATA
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPES
MAMMALS
MITOSIS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RADIATION EFFECTS
RADIOINDUCTION
RADIOISOTOPES
RIBOSIDES
RODENTS
SYNCHRONOUS CULTURES
URACILS
VERTEBRATES