G/sub 2/ cells: progression delay and survival

Journal Article · Tue May 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576246· OSTI ID:6816135

Schneiderman, M H; Schneiderman, G S

The progression of Chinese hamster ovary (CHO) G/sub 2/ cells into mitosis and their survival was measured after X-ray doses up to 4.0 Gy. S-phase cells were prevented from reaching mitosis by labeling with /sup 125/IUdR for 10 min prior to irradiation of the exponentially growing monolayer of cells. Mitotic cells, located past the radiation-induced division delay transition point, did not suffer a delay and were selected separately prior to the recovery of the G/sub 2/ cells. The results show that up to 400 min after radiation only 55% of the G/sub 2/ cells recovered after about 2.5 Gy; the progression delay of the G/sub 2/ cells that recovered was 52.5 min/Gy; and the survival curve D/sub 0/ for these cells, 2.45 Gy, indicated a radioresistant population.

• Research Organization: Thomas Jefferson Univ., Philadelphia, PA
• OSTI ID: 6816135
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 98:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
CELL DIVISION
CELL KILLING
CHO CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MITOSIS
MITOTIC DELAY
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RIBOSIDES
RODENTS
SURVIVAL CURVES
URACILS
VERTEBRATES
X RADIATION