Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

ESR of /sup 125/I labeled cells, x-irridiated cells, and x-irradiated ice at 4. 2K and above

Thesis/Dissertation ·
OSTI ID:6252049
Samples of L1210 mouse leukemia cells were irradiated at 77K with 3 Mev x-rays. ESR spectra were taken from 77K to 200K. The spectra for the x-irradiated samples were compared to that obtained for samples of /sup 125/I labeled L1210 cells, which were stored at either 77K or 80K for a maximum of 100 days. A sample of BP-8 sarcoma cells was x-irradiated at 4.2K, and ESR spectra were taken from 4.2K to 200K. A corresponding ESR study was conducted at 4.2K and above on /sup 125/I labeled cells, which were stored at 4.2K for thirteen days. Comparisons of the spectra observed for the x-irradiated cells and the /sup 125/I labeled cells revealed that the same major radical species were produced by both forms of irradiation. However, the relative concentrations of these radicals differed in the two cases. The major differences observed upon comparing the ESR spectra for /sup 125/I labeled cells and x-irradiated cells were: 1) A doublet, with a 17 gauss splitting, was observed to be more prominent in the spectra of the /sup 125/I labeled cells. 2) The relative concentration of hydroxyl radicals, in relation to the other radical species, was much less for the /sup 125/I labeled cells. 3) After warming to near 190K, the relative concentration of what is believed to be the thymine hydrogen addition radical was greater for the /sup 125/I labeled cells.
Research Organization:
Florida State Univ., Tallahassee (USA)
OSTI ID:
6252049
Country of Publication:
United States
Language:
English

Similar Records

ESR and ENDOR study of the effects of x-irradiation upon single crystals of L-asparagine monohydrate from 4. 2K to room temperature
Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984 · OSTI ID:6921504
ESR of /sup 125/IUdR-labeled cells and x-irradiated cells at 4. 2/sup 0/K and above
Journal Article · Mon Oct 01 00:00:00 EDT 1979 · Radiat. Res.; (United States) · OSTI ID:5733402
Early events elicited by Bombesin and structurally related peptides in quiescent Swiss 3T3 cells. I. Activation of protein kinase C and inhibition of epidermal growth factor binding
Journal Article · Tue Dec 31 23:00:00 EST 1985 · J. Cell Biol.; (United States) · OSTI ID:5669743

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CHEMISTRY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON SPIN RESONANCE
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONIZING RADIATIONS
ISOTOPES
LEUKEMIA
MAGNETIC RESONANCE
MAMMALS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOISOTOPES
RESONANCE
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TEMPERATURE DEPENDENCE
VERTEBRATES
X RADIATION