Ecotropic retroviruses of nonirradiated and irradiated C57BL/10. F mice

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:5732794

Soezer, A C

Mus musculus strains C57BL/10 and C57BL/10.F are congenic. Both strains carry a germline murine leukemia provirus, emv-2, on chromosome 8 but differ in the expression of ecotropic MuLV. Southern analysis of restriction endonuclease digested DNA and hybridization with a 400 base pair ecotropic virus specific probe were used to detect ecotropic proviruses. Restriction fragment length polymorphisms were used to identify unique proviral sequences, to determine partial restriction maps, and to examine the frequency and location of proviral integration of ecotropic MuLVs in the somatic cell DNA of these strains of mice. DNA from the spleens of six-month-old male B10.F mice contained multiple copies of ecotropic proviruses that were not detected in the B10 mice. Every B10.F mouse examined contained a provirus different from emv-2, this provirus was designated B10Fv. In addition to emv-2 and B10Fv, a variety of other novel proviruses were found in the DNA of B10.F splenocytes. These proviruses were integrated into many different sites in the DNA at an average frequency of two copies per cell. These novel proviruses may be recombinant viruses generated in these highly viremic animals. DNA from spleens and lymph nodes contained more copies of these proviruses than did DNA from nonlymphatic tissues (e.g. liver and kidney). To study the effects of radiation on the integration and appearance of novel acquired proviruses in the C57Bl/10.F mice, two-month-old male mice were exposed to a single dose of 1, 2, or 3 Gy of X-rays. Four months after the 2 and 3 Gy exposure there was an increase in the number of mice with enlarged spleens, incidence and severity of histopathological changes in the spleens, clonal expansion of cells containing a common provirus, and the number of spleens containing novel acquired proviruses.

• Research Organization: Tennessee Univ., Knoxville, TN (USA)
• OSTI ID: 5732794
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMES
DISEASES
DNA HYBRIDIZATION
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
GENE REGULATION
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
IONIZING RADIATIONS
ISOENZYMES
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
PARASITES
RADIATION EFFECTS
RADIATIONS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
X RADIATION