Comparative analysis of radiation- and virus-induced leukemias in BALB/c mice

Journal Article · Tue Jan 15 04:00:00 EST 1985 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(85)90449-0· OSTI ID:5663407

Newcomb, E W; Binari, R; Fleissner, E

Endogenous murine leukemia virus (MuLV) proviral copies were analyzed in thymomas induced in normal BALB/c (Fv-1b) and in Fv-1n congenic mice by X-irradiation. Both strains of mice developed leukemia with similar kinetics, indicating that N-tropism of endogenous MuLV was not a rate-limiting factor in development of disease. Southern blot analysis, using a probe specific for ecotropic virus and for ecotropic-specific sequences retained in pathogenic, env-recombinant viruses, showed that the majority of radiation leukemias lacked newly acquired, clonally integrated, proviruses. This was in contrast to virus-induced leukemias, which routinely exhibited several new proviral integration sites. When an internal proviral DNA restriction fragment was monitored, some radiation leukemias showed evidence of nonclonal infection, accounting for more frequent isolation of infectious virus from such leukemias. Differences in expression of T-cell surface antigens were found in X-ray-induced and virus-induced leukemias. All radiation leukemias were TL positive, whereas virus-induced leukemias were primarily negative for TL. Some differences were also found in Lyt-1 and Lyt-2 expression. The data as a whole suggest that, in the majority of cases, radiation leukemogenesis is not initiated by a viral route--that is, the sort of viral mechanism for which exogenous infection by known pathogenic MuLV is the paradigm.

• Research Organization: Memorial Sloan-Kettering Cancer Center, New York, NY
• OSTI ID: 5663407
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 140:1; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
COMPARATIVE EVALUATIONS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL NEOPLASMS
HEMIC DISEASES
IONIZING RADIATIONS
LEUKEMIA
LEUKEMIA VIRUSES
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
VERTEBRATES
VIRUSES
X RADIATION