Oncornaviruses produced by murine leukemia cells in culture

Journal Article · Thu Sep 01 04:00:00 EDT 1977 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(77)90152-0· OSTI ID:7289280

Nowinski, R C ^[1]; Hays, E F; Doyle, T; Linkhart, S; Medeiros, E; Pickering, R

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1. Fred Hutchinson Cancer Research Center, Seattle

Leukemia cells from mice of the AKR and C58 strains produced leukemia viruses (MuLV) in culture that failed to induce syncytia in the XC plaque assay (XC/sup -/), were poorly infectious for mouse embryo fibroblasts, and yielded low levels of infectious progeny upon the establishment of infection. In host range analysis the majority of these viruses were N-ecotropic, although one leukemia cell line also produced xenotropic MuLV. Viral isolates from five different leukemia cell lines were oncogenic upon inoculation into newborn AKR mice. In contrast, viruses that occurred in high titer in the tissues and sera of normal AKR mice were not oncogenic in vivo and in culture they efficiently induced (with N-ecotropism) syncytia in the XC plaque assay (XC/sup +/). These findings demonstrate that the transforming agent for spontaneous leukemogenesis differs in biological properties from the endogenous MuLV that is commonly observed in mice of high leukemic strains.

• OSTI ID: 7289280
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 81:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
HEMIC DISEASES
INFECTIVITY
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
RODENTS
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES
VIRUSES