Oncornaviruses produced by murine leukemia cells in culture
- Fred Hutchinson Cancer Research Center, Seattle
Leukemia cells from mice of the AKR and C58 strains produced leukemia viruses (MuLV) in culture that failed to induce syncytia in the XC plaque assay (XC/sup -/), were poorly infectious for mouse embryo fibroblasts, and yielded low levels of infectious progeny upon the establishment of infection. In host range analysis the majority of these viruses were N-ecotropic, although one leukemia cell line also produced xenotropic MuLV. Viral isolates from five different leukemia cell lines were oncogenic upon inoculation into newborn AKR mice. In contrast, viruses that occurred in high titer in the tissues and sera of normal AKR mice were not oncogenic in vivo and in culture they efficiently induced (with N-ecotropism) syncytia in the XC plaque assay (XC/sup +/). These findings demonstrate that the transforming agent for spontaneous leukemogenesis differs in biological properties from the endogenous MuLV that is commonly observed in mice of high leukemic strains.
- OSTI ID:
- 7289280
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 81:2; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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