Transient virus expression during murine leukemia induction by x-irradiation

Journal Article · Tue Feb 01 04:00:00 EST 1977 · J. Natl. Cancer Inst.; (United States)

OSTI ID:7312492

Haas, M

Most x-irradiation-induced thymomas in C57BL/6 mice are virus-free when assayed by immunofluorescence for the gs antigen (gsa) of murine leukemia virus (MuLV). Virus was induced transiently in bone marrow cells and later appeared in thymus cells. Six to 7 weeks post irradiation, thymocytes and bone marrow cells were MuLV gsa-negative and remained negative for the lifetime of most animals, whether or not they contracted overt leukemia. During the period when MuLV gsa-positive bone marrow cells were found, XC-positive syncytia-producing bone marrow cells were also found. Virus information was expressed, therefore, for a limited duration, long before any signs of leukemia in the animal were evident. MuLV gsa-positive thymocytes taken from mice 4 weeks after x-irradiation were cocultivated with a series of indicator cells. B-tropic virus, in addition to a xenotropic virus, was isolated from these cells. Ecotropic virus was not found in normal mouse thymocytes, in irradiated thymocytes a few days after termination of the X-irradiation sequence, or in most primary thymomas. All thymocytes produced only xenotropic virus in the cocultivation assays. Expression of the ecotropic virus was, therefore, transient, as assayed by immunofluorescence, XC syncytia formation, and virus isolation from MuLV gsa-positive thymus cells.

• Research Organization: Weizmann Inst. of Science, Rehovot, Israel
• OSTI ID: 7312492
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 58:2; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW CELLS
CARCINOGENESIS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
IONIZING RADIATIONS
IRRADIATION
LEUKEMOGENESIS
LYMPHATIC SYSTEM
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
ORGANS
PARASITES
PATHOGENESIS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
THYMUS
THYMUS CELLS
VERTEBRATES
VIRUSES
WHOLE-BODY IRRADIATION
X RADIATION