Radiation leukemia in C57BL/6 mice. II. Lack of ecotropic virus expression in the majority of lymphomas
The expression of endogenous ecotropic viruses in radiation-induced thymomas of C57BL/6 mice was examined. Competition radioimmunoassays for AKR MuLV were used for viral antigen expression. Three of 40 lymphomas had readily detectable ecotropic at levels of 95 to 689 ng/mg protein; the remainder of the tumors had no detectable (less than 1.0 ng/mg protein). Thirty thymomas were characterized by the presence of MuLV at levels of 1 to 10 ng/mg protein, levels that were comparable to those found in thymus extracts from age-matched, nonirradiated control. Ten tumors were characterized by having levels of 10 to 30 ng/mg protein. In one tumor significant levels of AKR MuLV were detectable. Since B-tropic and N-tropic viruses from C57BL/6 mice have glycoproteins indistinguishable from AKR MuLV and the N-tropic virus had a serologically identical to AKR MuLV, these results demonstrate that overt endogenous ecotropic virus expression is only rarely detectable in B-tropic virus was detectable in 2 of 40 thymomas and endogenous N-tropic virus was detectable in 1 of 40 thymomas. The lack of overt expression was also confirmed by cytotoxicity assays using monospecific antisera to these viral proteins. Radiation-induced lymphomas were also examined for the presence of reverse transcriptase after chromatography of tissue extracts on poly G-Sepharose. The results taken together demonstrate that overt endogenous ecotropic virus expression is only rarely detectable in radiation-induced thymomas of C57BL/6 mice.
- Research Organization:
- National Cancer Inst., Frederick, MD
- OSTI ID:
- 7323804
- Journal Information:
- J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 144:6; ISSN JEMEA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CARBOHYDRATES
DISEASES
DNA
ENZYMES
GLUCOPROTEINS
HEMIC DISEASES
ISOTOPE APPLICATIONS
LEUKEMIA
LYMPHATIC SYSTEM
LYMPHOMAS
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOINDUCTION
RNA
RODENTS
SACCHARIDES
THYMUS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES