Pathogenesis and virus content of lymphomas induced by pure ecotropic Graffi murine leukemia virus

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(85)90145-2· OSTI ID:5778997

Fischinger, P J; Dunlop, N M; Robey, W G

Tumors induced by wild type Graffi murine leukemia virus (Gi-MuLV) contained high titers of MuLV consisting of a predominant ecotropic (e)-MuLV and a scarcer titer recombinant (RM) MuLV component. Each of these was purified by biological cloning and examined for its envelope properties and leukemogenicity. Both the e- and the RM-MuLV's were single isolates and unique in terms of their neutralization profiles and peptide maps. The cloned E-Gi-MuLV was highly leukemogenic in C57Bl mice, inducing a very rapid lethal thymic lymphoma but no myeloid leukemia. e-Gi-MuLV also accelerated thymic lymphoma in AKR mice. The purified RM-MuLV did not induce any tumors. Infectious cell center (ICC) experiments of organs of mice inoculated with e-Gi-MuLV showed that virus replicated very rapidly and reached maximal titers in about one week in C57Bl mice. There was a highly preferential replication in the thymus of the animal so that this e-Gi-MuLV can be considered as thymotropic. Within two weeks after infection of mice, infected cells of the thymus also began to release low levels of a non-ecotropic MuLV. The rapid induction of lymphoma is compared to that induced by other e-MuLV's and their RM-MuLV's, and to the natural AKR-MuLV-associated disease. These findings are discussed in the content of prevailing theories on envelope gene rearrangements in the virus and in the proviral sequences in resulting tumors.

• Research Organization: National Cancer Institute, Bethesda, MD
• OSTI ID: 5778997
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 11:2; ISSN IOBPD
• Country of Publication: United States
• Language: English

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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUKEMIA VIRUSES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOMAS
MAMMALS
METHIONINE
MICE
MICROORGANISMS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PATHOGENESIS
PROTEINS
RADIOISOTOPES
RODENTS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES
VIRUSES