Molecular characterization of RadLV (radiation leukemia virus)

Thesis/Dissertation · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5742333

Grymes, R A

Following a course of a fractionated whole-body x-irradiation, a high percentage of C57BL/Ka mice develop thymic lymphomas. Radiation leukemia virus (RadLV) can be consistently isolated from these tumors. RadLV can induce identical lymphomas in unirradiated C57BL/Ka mice, and its role as the etiologic agent of these malignancies has been clearly documented. In order to characterize the viral genome at the molecular level, the unintegrated DNA intermediates of several RadLV isolates were prepared. Several different protocols were developed to optimize the yield and quality of restriction endonucleases, and cloned into a bacteriophage vector using recombinant DNA technology. RadLV is a general term used to describe a number of lymphomagenic viruses isolated from (1) primary radiation-induced lymphomas, (2) virus-induced lymphomas, and (3) established cell lines. The RadLV viruses replicate preferentially in the thymus and cause thymic lymphomas. Non-thymotropic, non-oncogenic, fibrotropic viruses can also be isolated from C57BL/Ka mice. Thus, a spectrum of biological activities can be seen among the C57BL/Ka mouse retroviruses. This dissertation reports the molecular cloning of three of the RadLV viruses. They are: (1) RadLV/VL/sub 3/ and (2) RadLV/RL/sub 10/, both lymphomagenic viruses, and (3) RadLV/RL/sub 15/, a non-oncogenic isolate. The generation of restriction maps from both the cloned DNAs and the unintegrated viral DNA intermediates is described. These maps are compared with those of other C57BL/Ka murine retroviruses, and with published maps of other murine leukemia viruses.

• Research Organization: Stanford Univ., CA (USA)
• OSTI ID: 5742333
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
CLONING
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GENETIC MAPPING
IONIZING RADIATIONS
IRRADIATION
LEUKEMIA VIRUSES
LYMPHOMAS
MAMMALS
MAPPING
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
RADIATIONS
RADIOINDUCTION
RODENTS
VERTEBRATES
VIRUSES
WHOLE-BODY IRRADIATION
X RADIATION