Evidence for a new form of retroviral env transcript in leukemic and normal mouse lymphoid cells

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · J. Virol.; (United States)

OSTI ID:5653242

Boccara, M; Souyri, M; Magarian, C; Stavnezer, E; Fleissner, E

Murine leukemia virus-related RNA species were examined in a set of radiation-induced T-cell leukemias from BALB/c mice. No evidence was found for linkage of viral long terminal repeat-derived (U5) sequences to information of host origin. A novel class of 2-kilobase (kb) env-related transcripts, about 1kb shorter than normal viral env messenger, was found in all the leukemias. All of the 2-kb transcripts contained sequences homologous to the xenotropic virus-related env sequences in the Friend spleen focus-forming virus, representing the N-terminal portion of gp70. In two of the leukemias, these transcripts were found to contain both ecotropic p15E and U3 sequences in addition to the xenotropic gp70-related sequence. These two leukemias, but not others in which ecotropic sequences were absent from the 2-kb RNA, harbored several copies of a specific class of env recombinant proviruses. These proviruses possessed full-size env genes and were submethylated, as shown by SmaI and XmaI digests of proviral DNA. Low levels of 2-kb RNA were found in normal thymocytes from strains BALB/c, AKR, and 129 but not from congenic 129 GIX- mice. It is possible that the 2-kb RNA may originate by a novel splicing step that removes portions of the gp70 and p15E sequences from full-length env transcripts.

• Research Organization: Memorial Sloan-Kettering Cancer Center, Cornell University Graduate School of Medical Sciences, New York, New York
• OSTI ID: 5653242
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 48:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
DISEASES
GENES
HEMIC DISEASES
LEUKEMIA
LEUKEMIA VIRUSES
MAMMALS
MESSENGER-RNA
MICE
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RADIOINDUCTION
RNA
RODENTS
SOMATIC CELLS
THYMUS CELLS
TRANSCRIPTION
VERTEBRATES
VIRUSES