Specific hybridization probes demonstrate fewer xenotropic than mink cell focus-forming murine leukemia virus env-related sequences in DNAs from inbred laboratory mice

Journal Article · Thu May 01 04:00:00 EDT 1986 · J. Virol.; (United States)

OSTI ID:6204244

O'Neill, R R; Khan, A S; Hoggan, D; Hartley, J W; Martin, M A; Repaske, R

The authors have derived hybridization probes from analogous 100-base-pair segments located within the N-terminal region of gp70 coding sequences which differentiate xenotropic from mink cell focus-forming (MCF)-related murine leukemia virus (MuLV) DNAs. The MCF probe annealed to the integrated proviruses of all six MCF MuLV isolates tested; the xenotropic probe hybridized to the DNAs of all four xenotropic proviral isolates examined. No cross-hybridization was observed, and neither probe reacted with the env segments of amphotropic or ecotropic MuLV DNAs. Southern blot analysis of HindIII- or EcoRI-digested genomic DNAs from a variety of inbred laboratory mice demonstrated the presence of more MCF- than xenotropic MuLV-related segments in every strain tested.

• Research Organization: National Institute of Allergy and Infectious Diseases, Bethesda, MD (USA)
• OSTI ID: 6204244
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 58:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTROPHORESIS
HYBRIDIZATION
ISOTOPES
LEUKEMIA VIRUSES
LIGHT NUCLEI
MAMMALS
MICE
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RESPIRATORY TRACT CELLS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES