Retinoid inhibition of in vitro invasion of human amnion basement membrane by human tumor cells

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5718685

Fazely, F

The effects measured were the inhibition of tumor cell migration through the basement membrane (BM) and tumor cell degradative enzyme activity on {sup 3}H-proline labeled collagenous and non collagenous components of the BM. The human lung carcinoma A549 or the human Ewing's sarcoma TC-106 cell lines treated with retinoids for two days were incubated on the BM in the absence of retinoids. A dose-dependent inhibition of cell invasion was produced by retinoids. Among the retinoids tested the most powerful was retinol acetate which inhibited invasion by 50% of A549 cells at a concentration of 0.09 {mu}g/ml, and TC-106 cells at 0.08 {mu}g/ml. Retinol acetate inhibited A549 and TC-106 cell growth by approximately 50% at levels almost 100-fold higher than those needed for antiinvasive activity. Retinol acetate was about 20 times more potent than retinoic acid and 30 times more than retinol palmitate. Furthermore, A549 cells treated with retinol acetate, under conditions whereby an anti-invasive state was induced,showed an increase in the number of cellular retinoic acid binding proteins (CRABP), a decrease in the activity of type IV collagenase and ectosialyltransferase, and no change in the activity of transglutaminase.

• Research Organization: Akron Univ., OH (USA)
• OSTI ID: 5718685
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ESTERS
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IN VITRO
INHIBITION
ISOTOPE APPLICATIONS
LUNGS
MAMMALS
MAN
MEMBRANES
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PROLINE
PYRROLES
PYRROLIDINES
RESPIRATORY SYSTEM
RETINOIC ACID
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES