Retinoid-binding proteins in seven new retinoblastoma cell lines: a comparison with Y-79

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7246537

Alvarez, R A; Fong, S L; Landers, R A; Liou, G I; Gallie, B L; Bridges, C D.B.

Studies of retinoid-binding proteins in retinoblastoma (Rb) tumor cells have been confined to Y-79 and WERI cell lines. They report on 7 new established Rb lines and compare amounts of cytosol retinol and retinoic acid-binding proteins (CRBP, CRABP) and interstitial retinol-binding protein (IRBP) to those found in Y-79. The new Rb lines synthesized and secreted IRBP (M/sub r/ = 135K) into the culture medium. The amount as determined by immunoblotting ranged from 54.8 - 117.0 pmol x 10/sup -8/ cells expressed in equivalents of standard b-IRBP. The cell cytosols were examined for CRBP and CRABP after incubation with (/sup 3/H)-retinoid and chromatography on a TSK-250 size-exclusion column. Specific binding was abolished at M/sub r/ = 16K by addition of p-chloromercuribenzene sulfonate. CRBP averaged 2.3 +/- 2.7 RO x mg protein/sup -1/. CRABP averaged 1.0 +/- 1.2 pmol RA x mg protein /sup -1/. Two lines (247,383) contained no measurable amounts of CRBP or CRABP. In contrast, Y-79 cells were strikingly different in that the amount of IRBP was only 1% of the new lines. This figure was not measurably enhanced by the addition of retinoic acid to the growth medium. CRABP was 9 times higher than the new lines and CRBP was present in similar amounts.

• Research Organization: Baylor College of Medicine, Houston, TX
• OSTI ID: 7246537
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOSYNTHESIS
CARBOXYLIC ACID ESTERS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ESTERS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PROTEINS
RETINOIC ACID
SECRETION
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS