Antigenotoxic effects of retinoids
In order to test the hypothesis that retinoids are not genotoxic in mammalian cells, and that retinoids are capable of modulating 7,12-dimethyl-benz(a)anthracene (DMBA) genotoxicity in mammalian cells, the ability of retinol and retinoic acid to modulate induction of unscheduled DNA synthesis (UDS) in hepatocytes obtained from rats in the presence and absence of DMBA, ethyl methane-sulfonate (EMS) and ultraviolet light (UV) was quantitated. The ability of retinol and retinoic acid to modulate mutagenicity at the hypoxanthine-guaine phosphoribosyl transferase (HGPRT) locus of Chinese hamster ovary (CHO) cells in the presence and absence of EMS without exogenous metabolic activation, and in the presence and absence of DMBA with exogenous metabolic activation was also investigated. The ability of retinol and retinoic acid to induce cytotoxicity measured as lacatate dehydrogenase (LDH) release in isolated primary rat hepatocytes was also tested.
- Research Organization:
- Arkansas Univ., Fayetteville, AR (USA)
- OSTI ID:
- 5345478
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZANTHRACENE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACID ESTERS
CHO CELLS
CONDENSED AROMATICS
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYDROCARBONS
INHIBITION
LIVER CELLS
MAMMALS
METABOLIC ACTIVATION
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
RADIATION EFFECTS
RADIATIONS
RATS
RETINOIC ACID
RODENTS
SOMATIC CELLS
SULFONATES
TOXICITY
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES