Retinoid inhibition of in vitro invasion of human amnion basement membrane by human tumor cells

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7188292

Fazely, F; Ledinko, N; Smith, D J

The biological activity of retinoids was assayed in an in vitro quantitative assay of human tumor cell invasion using human amnion basement membrane (BM). The effects measured were the inhibition of tumor cell migration through the BM and tumor cell degradative enzyme activity on /sup 14/C-proline labeled collagenous and noncollagenous components of the BM. The human lung carcinoma A549 or the human Ewing's sarcoma TC-106 cell lines treated with retinoids for two days were incubated on the BM in the absence of retinoids. A dose-dependent inhibition of cell invasion was produced by retinoids. Among the retinoids tested, the most powerful was retinol acetate which inhibited invasion by 50% of A549 cells at a concentration of 0.009 ..mu..g/mL, and of TC-106 cells at 0.07 ..mu..g/mL. Retinol acetate inhibited A549 and TC-106 cell growth by approximately 50% at levels over 100-fold higher than those needed for antiinvasive activity. Retinol acetate was about 20 times more potent than retinoic acid and 30 times more potent than retinol palmitate. The model system will be useful for investigating antiinvasive activity of other retinoids as well as other compounds.

• Research Organization: Univ. of Akron, OH
• OSTI ID: 7188292
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACID ESTERS
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ESTERS
IN VITRO
INHIBITION
LABELLED COMPOUNDS
LUNGS
MAMMALS
MAN
MEMBRANES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RESPIRATORY SYSTEM
RETINOIC ACID
SARCOMAS
TUMOR CELLS
VERTEBRATES