Impairment of aminopyrine clearance in aspirin-damaged canine gastric mucosa

Journal Article · Thu Sep 01 04:00:00 EDT 1983 · Gastroenterology; (United States)

OSTI ID:5714334

Miller, T A; Henagan, J M; Loy, T M

Using an in vivo canine chambered stomach preparation, the clearance of (/sup 14/C)aminopyrine across mucosa when intravenously infused and the back-diffusion of this substance from gastric lumen to mucosa when topically applied to gastric epithelium were evaluated in aspirin-damaged gastric epithelium. In mucosa damaged by either 20 mM or 40 mM aspirin, the recovery of (/sup 14/C)aminopyrine, when topically mixed with acid (pH . 1.1) perfusate solution, was not significantly different from nondamaged control mucosa. In addition, the degree of ''trapping'' of this substance from back-diffusion was not different in damaged mucosa from that observed in nondamaged epithelium. In contrast, when (/sup 14/C)aminopyrine was intravenously infused, its clearance was significantly impaired in aspirin-damaged mucosa when compared with control studies, as evidenced by the increased ''trapping'' of this substance in injured epithelium. These findings indicate that movement of aminopyrine from plasma to gastric lumen is impaired in damaged epithelium, making the aminopyrine clearance technique an unreliable method to accurately measure absolute gastric blood flow in this experimental setting.

• Research Organization: Department of Surgery, University of Texas Medical School, Houston
• OSTI ID: 5714334
• Journal Information: Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 85:3; ISSN GASTA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLSALICYLIC ACID
ANALGESICS
ANIMALS
ANTIPYRETICS
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CLEARANCE
DIGESTIVE SYSTEM
DOGS
DRUGS
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
IN VIVO
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MUCOUS MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRAZOLES
STOMACH
TRACER TECHNIQUES
VERTEBRATES