Calcium secretion in canine tracheal mucosa
Calcium (Ca) affects many cellular functions of the respiratory tract mucosa and might alter the viscoelastic properties of mucus. To evaluate Ca homeostasis in a respiratory epithelium we investigated transport of Ca by the canine tracheal mucosa. Mucosal tissues were mounted in Ussing-type chambers and bathed with Krebs-Henseleit solution at 37 degrees C. Unidirectional fluxes of 45Ca were determined in tissues that were matched by conductance and short-circuit current (SCC). Under short-circuit conditions there was a significant net Ca secretion of 1.82 +/- 0.36 neq . cm-2 . h-1 (mean +/- SE). Under open-circuit conditions, where the spontaneous transepithelial potential difference could attract Ca toward the lumen, net Ca secretion increased significantly to 4.40 +/- 1.14 compared with 1.54 +/- 1.17 neq . cm-2 . h-1 when the preparation was short-circuited. Addition of a metabolic inhibitor, 2,4-dinitrophenol (2 mM in the mucosal bath), decreased tissue conductance and SCC and slightly decreased the unidirectional movement of Ca from submucosa to lumen. Submucosal epinephrine (10 microM) significantly enhanced Ca secretion by 2.0 +/- 0.63 neq . cm-2 . h-1. Submucosal ouabain (0.1 mM) failed to inhibit Ca secretion. The data suggest that canine tracheal mucosa secretes Ca; this secretory process is augmented by epinephrine or by the presence of a transepithelial potential difference as found under in vivo conditions.
- Research Organization:
- Univ. of Illinois College of Medicine, Chicago
- OSTI ID:
- 6331115
- Journal Information:
- J. Appl. Physiol.; (United States), Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENALINE
BIOLOGICAL EFFECTS
CALCIUM
MEMBRANE TRANSPORT
SECRETION
NITROPHENOL
OUABAIN
CALCIUM 45
DOGS
ELECTRIC CONDUCTIVITY
EPITHELIUM
HOMEOSTASIS
METABOLISM
MUCOUS MEMBRANES
RESPIRATORY SYSTEM
TRACHEA
ADRENAL HORMONES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRICAL PROPERTIES
ELEMENTS
EVEN-ODD NUCLEI
GLYCOSIDES
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MEMBRANES
METALS
NEUROREGULATORS
NITRO COMPOUNDS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PHYSICAL PROPERTIES
RADIOISOTOPES
STEROID HORMONES
STROPHANTHINS
SYMPATHOMIMETICS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques