DNA sequence mapping by fluorescence in situ hybridization

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850180410· OSTI ID:5688732

Brandriff, B F; Gordon, L A; Trask, B J ^[1]

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1. Univ. of California, Livermore (United States)

Various types of DNA probes, such as total genomic DNA, repetitive sequences, unique sequences, and composites of chromosome-specific DNA probes, can be used with fluorescence in situ hybridization (FISH) techniques to address research questions having to do with localization, mapping, and distribution of DNA in situ. FISH involves the formation of a heteroduplex between such DNA probes and chromatin targets on a microscope slide, which can be visualized with fluorescent reporter molecules. Three chromatin targets - metaphase chromosomes, somatic interphases, and zygote interphases - offer increasingly extended states of chromatin which can be strategically selected, individually or in combination, to address specific research questions of interest.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5688732
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:4; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMATIN
CONNECTIVE TISSUE CELLS
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
GENE MUTATIONS
GENETIC MAPPING
HYBRIDIZATION
LEUKOCYTES
LYMPHOCYTES
MAPPING
MATERIALS
MOLECULAR BIOLOGY
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RECOMBINANT DNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS