Sequence independent amplification of DNA
Patent
·
OSTI ID:597114
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.
- Research Organization:
- Univ. of Chicago, IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
- DOE Contract Number:
- FG02-86ER60408
- Assignee:
- Arch Development Corp., Chicago, IL (United States)
- Patent Number(s):
- US 5,731,171/A/
- Application Number:
- PAN: 8-096,637; CNN: Grant CA49133;CA42557;CA40046
- OSTI ID:
- 597114
- Resource Relation:
- Other Information: PBD: 24 Mar 1998
- Country of Publication:
- United States
- Language:
- English
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