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Sequence independent amplification of DNA

Patent ·
OSTI ID:871434
 [1]
  1. Chicago, IL
+ Show Author Affiliations
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.
Research Organization:
Argonne National Laboratory (ANL), Argonne, IL
DOE Contract Number:
FG02-86ER60408
Assignee:
Arch Development Corp. (Chicago, IL)
Patent Number(s):
US 5731171
OSTI ID:
871434
Country of Publication:
United States
Language:
English

References (10)

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction journal January 1986
Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer journal July 1992
A method for the rapid sequence-independent amplification of microdissected chromosomal material journal August 1992
PCR amplification of megabase DNA with tagged random primers (T-PCR) journal January 1993
Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification journal March 1989
YAC mapping by FISH using Alu-PCR-generated probes journal July 1992
Chromosomal assignment of human YAC clones by fluorescence in Situ hybridization: Use of single-yeast-colony PCR and multiple labeling journal September 1992
Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: identification of clones associated with a disease locus. journal July 1991
PCR amplification and analysis of yeast artificial chromosomes journal August 1992
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification journal July 1992

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Related Subjects

/435/
allows
also--with
amounts
amplification
amplification procedure
amplified
characteristics
chromosomal
chromosomal material
chromosomes
construction
dna
efficiency--in
example
fish
fluorescent
hybridization
independent
independent amplification
interphase
interphase nuclei
knowledge
localize
material
metaphase
method
method allow
method allows
microdissected
minute
minute amounts
nuclei
priori
probes
procedure
quality
rapid
reliable
requirements
sequence
sequence-independent
sia
situ
situ hybridization
sources
various
various sources
yacs