Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model

Journal Article · Fri Sep 04 04:00:00 EDT 1992 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.1388286· OSTI ID:7007034

Engh, G van den; Trask, B J ^[1]; Sachs, R ^[2]

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1. Lawrence Livermore National Lab., Livermore, CA (United States)
2. Univ. of California, Berkeley (United States)

The folding of chromatin in interphase cell nuclei was studied by fluorescent in situ hybridization with pairs of unique DNA sequence probes. The sites of DNA sequences separated by 100 to 2000 kilobase pairs (kbp) are distributed in interphase chromatin according to a random walk model. This model provides the basis for calculating the spacing of sequences along the linear DNA molecule from interphase distance measurements. An interphase mapping strategy based on this model was tested with 13 probes from a 4-megabase pair (Mbp) region of chromosome 4 containing the Huntington disease locus. The results confirmed the locations of the probes and showed that the remaining gap in the published maps of this region is negligible in size. Interphase distance measurements should facilitate construction of chromosome maps with an average marker density of one per 100 kbp, approximately ten times greater than that achieved by hybridization to metaphase chromosomes.

• OSTI ID: 7007034
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 257:5075; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
CELL CYCLE
CELL NUCLEI
CHROMATIN
CHROMOSOMES
DISEASES
DNA HYBRIDIZATION
DNA SEQUENCING
FLUORESCENCE
GENETIC MAPPING
HYBRIDIZATION
LUMINESCENCE
MAPPING
MATHEMATICAL MODELS
NERVOUS SYSTEM DISEASES
STRUCTURAL CHEMICAL ANALYSIS