Evaluation of radioimmunoassay and in vitro colony assay techniques for determination of colony-stimulating factor and inhibitory activity in murine serum and tissue

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Blood; (United States)

OSTI ID:5682216

Boegel, F; Waheed, A; Shadduck, R K

These studies have evaluated a newly developed radioimmunoassay (RIA) for the measurement of colony-stimulating factor (CSF) in murine serum and other biological fluids. The routine in vitro agar gel bioassay for CSF is influenced by high molecular weight serum lipoproteins and low molecular weight tissue-derived materials that are inhibitory to colony formation. Studies with normal serum revealed that in all cases, the levels of CSF detected by the RIA were equal to or greater than levels measured by the bioassay. Dose curves with varying quantities of serum had linear responses in the RIA but not the colony assay. Using Sephadex G-150 chromatography, the murine serum was separated into CSF active and CSF inhibitory peaks as determined by bioassay. Evaluation of these fractions by RIA indicated that the assay was unaffected by the serum inhibitors. Likewise, experiments with tissue lysates indicated that the RIA was not influenced by the low molecular weight tissue inhibitors. Instead, the radioimmunoassay revealed low levels of CSF that were not detectable by bioassay. These observations indicate that the RIA is superior to the bioassay for the estimation of CSF in murine serum and other biologic materials.

• Research Organization: Univ. of Pittsburgh School of Medicine, PA
• OSTI ID: 5682216
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 58:6; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOASSAY
BLOOD SERUM
CELL PROLIFERATION
COLONY FORMATION
COMPARATIVE EVALUATIONS
DATA
EXPERIMENTAL DATA
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MICE
NUMERICAL DATA
RADIOASSAY
RADIOIMMUNOASSAY
RODENTS
SENSITIVITY
TRACER TECHNIQUES
VERTEBRATES