Colony-stimulating factor (CSF) radioimmunoassay: detection of a CSF subclass stimulating macrophage production
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Colony-stimulating factors (CSFs) stimulate the differentiation of immature precursor cells to mature granulocytes and macrophages. Purified /sup 125/I-labeled murine L cell CSF has been used to develop a radioimmunoassay (RIA) that detects a subclass of CSFs that stimulates macrophage production. Murine CSF preparations that contain this subclass of CSF compete for all of the CSF binding sites on anti-L cell CSF antibody. With the exception of mouse serum, which can contain inhibitors of the bioassay, there is complete correspondence between activities determined by RIA and those determined by bioassay. The RIA is slightly more sensitive than the bioassay, detecting approximately 0.3 fmol of purified L cell CSF. It can also detect this subclass of CSF in chickens, rats, and humans. In the mouse, the subclass is distinguished from other CSFs by a murine cell bioassay dose-response curve in which 90% of the response occurs over a 10-fold (rather than a 100-fold) increase in concentration, by stimulating the formations of colonies contaning a high proportion of mononuclear (rather than granulocytic) cells, and by certain physical characteristics.
- Research Organization:
- Albert Einstein College of Medicine, Bronx, NY
- OSTI ID:
- 6137407
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 76:6; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evaluation of radioimmunoassay and in vitro colony assay techniques for determination of colony-stimulating factor and inhibitory activity in murine serum and tissue
Isolation of a murine osteoclast colony-stimulating factor
Purified murine granulocyte/macrophage progenitor cells express a high-affinity receptor for recombinant murine granulocyte/macrophage colony-stimulating factor
Journal Article
·
Mon Nov 30 23:00:00 EST 1981
· Blood; (United States)
·
OSTI ID:5682216
Isolation of a murine osteoclast colony-stimulating factor
Journal Article
·
Tue Oct 01 00:00:00 EDT 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5826717
Purified murine granulocyte/macrophage progenitor cells express a high-affinity receptor for recombinant murine granulocyte/macrophage colony-stimulating factor
Journal Article
·
Thu Dec 31 23:00:00 EST 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6142659
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOASSAY
BLOOD FORMATION
CARBOHYDRATES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLUCOPROTEINS
IMMUNE SERUMS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MACROPHAGES
MAMMALS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
STIMULATION
TRACER TECHNIQUES
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOASSAY
BLOOD FORMATION
CARBOHYDRATES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLUCOPROTEINS
IMMUNE SERUMS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MACROPHAGES
MAMMALS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
STIMULATION
TRACER TECHNIQUES
VERTEBRATES