Development and characterization of antiserum to murine granulocyte-macrophage colony-stimulating factor

Journal Article · Thu May 15 00:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:5006572

Mochizuki, D Y; Eisenman, J R; Conlon, P J; Park, L S; Urdal, D L

The expression in yeast of a cDNA clone encoding murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has made possible the purification of large quantities of this recombinant protein. Rabbits immunized with pure recombinant GM-CSF generated antibodies that were shown to be specific for both recombinant GM-CSF and GM-CSF isolated from natural sources. Other lymphokines, including IL 1..beta.., IL 2, IL 3, and recombinant human GM-CSF did not react with the antiserum. The antiserum together with recombinant GM-CSF that had been radiolabeled with /sup 125/I to high specific activity, formed the foundation for a rapid, sensitive, and quantitative radioimmunoassay specific for murine GM-CSF. Furthermore, the antiserum was found to inhibit the biologic activities of GM-CSF as measured in both a bone marrow proliferation assay and a colony assay, and thus should prove to be a useful reagent for dissecting the complex growth factor activities involved in murine hematopoiesis.

• Research Organization: Immunex Corp., Seattle, WA
• OSTI ID: 5006572
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:10; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY FLUIDS
BONE MARROW CELLS
CARBOHYDRATES
CELL CULTURES
CELL PROLIFERATION
CHEMICAL PREPARATION
CLONE CELLS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
ELECTRON CAPTURE RADIOISOTOPES
FUNGI
GLUCOPROTEINS
GROWTH FACTORS
IMMUNE SERUMS
IMMUNOASSAY
IMMUNOLOGY
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOKINES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PLANTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
STIMULATION
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
YEASTS